Isolation of Heavy Basophils in Carp Peripheral Blood by Percoll Density Gradient Centrifugation
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Evaluation of three substitutes for Percoll in sperm isolation by density gradient centrifugation.
Silane-coated silica particle solutions (ISolate(TM) and PureSperm)TM)) and iodixanol (OptiPrep(TM)) were compared to polyvinylpyrrolidone (PVP)-coated silica particles (Percoll(TM)) in their efficacy to recover spermatozoa by gradient centrifugation for use in assisted reproductive procedures. Efficacy was assessed in terms of percentages of sperm recovery, sperm vitality and motility, normal ...
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A procedure for isolating spinach (Spinacia oleracea L.) leaf peroxisomes in 0.25 molar sucrose solution by Percoll density gradient centrifugation followed by removal of the Percoll by washing and centrifugation was established. The preparation contains more than 90% peroxisomes as intact organelles with no detectable chlorophyll or cytochrome oxidase contamination. The peroxisomes are stable ...
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The isolation of Cowdria ruminantium by means of Percoll density gradient centrifugation permits the recovery of partially purified viable populations of the organism possessing distinctly different densities. These conclusions are based upon results of analyses of density fractions by intravenous inoculation into sheep, protein determination, electron microscopy and enzyme-linked immunosorbent...
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Analysis of cellular effector function(s) often requires their isolation from other cellular types. Cell separatory techniques could mask, or select out, clinically important functional lesions. We examined differences in canine peripheral blood neutrophil functions, i.e. migration and H202 production, following two commonly used cell separation techniques: isotonic lysis or density gradient (P...
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In this study, potential of the Percoll density gradient centrifugation method for separation of bovine red bold cells (RBCs) infected with each merozoite type of Babesia ovata was examined. By the Percoll density gradient centrifugation, bovine RBCs infected with active forms of B. ovata merozoites were essentially collected from one of the layers. Moreover, this method made it possible to eli...
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ژورنال
عنوان ژورنال: NIPPON SUISAN GAKKAISHI
سال: 1988
ISSN: 0021-5392,1349-998X
DOI: 10.2331/suisan.54.1257